Zinc chloride protects against streptozotocininduced. Streptozotocin produces diabetes by destruction of the insulinsecreting cells of the pancreas arison et al. The activities of sod and cat were reduced in liver and kidney tissues of diabetic rats. In the rat models of stz induced diabetes, male rats at 8 weeks of age 200250 g are starved for 16 h and injected once into the tail vein with stz sd 55 mgkg, wky 60 mgkg, shr 45 mgkg in sodium citrate buffer 1 mlkg cooper et al.
Induction of diabetes by streptozotocin in rats ncbi nih. These findings suggest that the redox abnormality resulting from diabetes increases oxidative stress which facilitates andor causes endotheliumdependent contractions. Neonatal streptozotocininduced rat model of type 2 diabetes. Stz is transported into bcells via the glucose transporter glut2 and causes dna damage leading to increased activity of polyadpribose polymerase parp1 to repair dna. Effect of bauhinia holophylla treatment in streptozotocin. Methods animal models this prospective randomized controlled study was carried at sultan qaboos university in muscat, oman between january 20 and july 2014. The n stz model with alteration of dose and day of stz injection exhibits various stages of type 2 diabetes mellitus such as impaired glucose tolerance, and mild, moderate and severe glycemia. These results are in total agreement in previous studies employing the stz induced diabetic rats. These results suggested that the compound might increase the biosynthesis of gsh or reduce the oxidative stress or both. The effect of streptozotocininduced diabetes in rats. Effects of stzinduced diabetes on the relative weights of kidney.
Free radicalsinduced lipid peroxidation of the membrane could increase the rigidity of. Apr 05, 2018 studies of diabetes mellitus in the streptozotocin rat model suggest that sexual dysfunctions may result from diabetes induced alterations of the neuroendocrinereproductive tract axis. At lower doses, stz induced diabetes is not stable, since spontaneous recovery occurs. Streptozotocinnicotinamideinduced diabetes in the rat. In this study, we investigated fibromuscular changes in the corpus cavernosum of rats with streptozotocin induced diabetes. Hypoglycemic, hypolipidemic and antioxidant effects of green. This project has assessed cardiovascular, ocular and neuropathic changes over a period of 24 weeks post stz treatment in rats. The mechanisms of alloxan and streptozotocininduced. The use of rats and all the experimental procedures were approved by the animal ethical committee at sultan qaboos university squ. Amelioration of streptozotocin induced diabetes in rats by. Nutrients free fulltext effects of tempeh fermentation. The stz was dissolved in a citrate buffer solution 0. Diabetes was induced through the injection of male rats with a single intraperitoneal dose 45 mgkg of streptozotocin. In the diabetic group, diabetes was induced by a single intraperitoneal injection of freshly dissolved stz in a 0.
Treatment with ellagic acid significantly p jun 30, 2016. Effect of streptozotocin on glucose levels in albino wister rats. Develop treatments to diabetes induced chronic wounds diabetes pathophysiology study of diabetes side effects model readouts blood glucose level body weight insulin tolerance test serum insulin levels methodology adult nude female rats ranging from 365430 g were divided into two groups. Protective nature of mangiferin on oxidative stress and. Cnp was found to be released from endothelial cells of the perfused rat mesenteric bed in response to endotheliumdependent vasodilators such as acetylcholine. After induction of diabetes, diabetic mice were randomly divided into five. Streptozotocin diabetesinduced changes in aorta, peripheral. However, the choice of an experimental model of diabetes type 1 or 2 is largely determined by the purpose of the. Pdf effect of streptozotocininduced type 1 diabetes. In the present study streptozotocin dose was as 50mgkg body weight in study of zafar16, m et al used 50mgkg bw stz dose, in study of mozaffari et al23 used 90 mgkg body weight.
The full text of this article is available as a pdf 180k. The animals then receive injections of streptozotocin to induce insulindependent diabetes mellitus. Treatment of young adult rats with streptozotocin stz produces a diabetic state that is characterized by loss of weight, polydipsia, polyuria, glucosuria, polyphagia, hypoinsulinaemia and hyperglycaemia hakim et al. Summary in order to determine whether diabetic cardiomyopathy in rats is associated with altered contractile proteins, male and female rats were made diabetic with intravenous streptozotocin stz.
For severe diabetes induction, stz is administered at 4050 mgkg body weight intravenously or intraperitoneally during adulthood. Experimentally induced diabetes in male syrian hamsters also reduced the peak pineal gland melatonin content, which was then restored to normal by insulin injections. After the development of diabetes, diabetic rats were orally administered daily with 1ml of with fenugreek received. Cerebellar synaptopathy in streptozotocininduced diabetic. Oxygenderived free radicals mediate endotheliumdependent. A comparison with the chronic changes in human diabetes will indicate whether the stz diabetic rat is an adequate model of the human disease. This study sought to evaluate the antihyperglycemic, antihyperlipidemic and antioxidant effect of c. Administration of both streptozotocin stz and nicotinamide na has been proposed to induce experimental diabetes in the rat. This suggested the drugs use as an animal model of diabetes, and as a medical treatment for cancers of the beta cells. Detailed in this unit are protocols for producing stz. Calcium atpase activity of cardiac actomyosin was significantly decreased after 1 week of diabetes and was depressed by 60% by 2 weeks. Materials and methods animals forty 8weekold male spraguedawley rats weighing 200 to 250 g were randomly distributed into 2 groups normal. Show full abstract streptozotocin stz induced diabetic rats. Oxidative stress plays an important role in the progression of diabetes complications.
Our investigation was performed to better define the effects of shortterm hyperglycemia on rat epididymal sperm quantity, quality and transit time, using both natural mating and artificial in utero insemination protocols. Changes in hormone sensitivity of the adenylate cyclase signaling system in the testicular tissue of rats with neonatal streptozotocin induced diabetes. Pdf effects of melatonin on streptozotocininduced diabetic. Objective streptozotocin stz is the most widely used diabetogenic agent in animal models of islet transplantation.
At cbi, this model is induced in male bn rats by stz administration. Ozone treatment reduces blood oxidative stress and. Bauhinia holophylla leaves treatment on the streptozotocin induced diabetic rats. Zoology and wildlife conservation health aspects diabetes therapy methods. The animals were kept on a standard diet with free access to water for 4 months. Control rats received injections of the buffer only. Methods diabetes was induced in 8 week old male wistar rats.
Pdf induction of diabetes by streptozotocin in rats. An insight into its applications to induce diabetic complications volume. For induction of experimental diabetes in male adult rats weighted 250300 grams 7590 days, 60mgkg of. Streptozotocininduced diabetic models in mice and rats. Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell. Pdf streptozotocininduced diabetes reduces brain serotonin. Multiple administrations of lowdose stz are employed to produce diabetic mice. A hydroalcoholic extract of gymnema sylvestre leaves 100 mgkg b w day was tested for its biochemical and pathomorphological effects in streptozotocin induced diabetes in rats.
The streptozotocin induced stz model of diabetes induced by stz administration in rats serves as a validated and reproducible model of retinal hypervascularity in brown norway rats bn. Hypoglycemic, hypolipidemic and antioxidant effects of. Effects of balloon injury on neointimal hyperplasia in. Ozone treatment reduces blood oxidative stress and pancreas. The superoxide dismutase sod and catalase cat are two key scavenging enzymes that eliminate the toxic free radicals induced by stz. Diabetes is a chronic disease that can affect almost all of the body organs, including male and female reproductive systems.
Depending on the animal strain, dose, route of drug administration, and the life period in which stz is administered in rats, severe diabetes blood glucose superior to 200300 mgdl 7177 or mild diabetes glycemia between 120 and 200300 mgdl are generated 68, 7881. In all, 16 f344 rats had diabetes induced by streptozotocin stz, and were divided into insulintreated. Pdf induction of diabetes mellitus in rats using intraperitoneal. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. The effects of metformin on stereological and ultrastructural.
The effect of streptozotocininduced diabetes in rats on. However, the immunomodifying effects of stz and the ensuing hyperglycemia on lymphocyte subsets, particularly on t regulatory cells tregs, remain poorly understood. The present experiments were designed to study the contribution of oxygenderived free radicals to endotheliumdependent contractions in femoral arteries of rats with streptozotocin induced diabetes. Streptozotocin induced diabetes in laboratory animals presents an experimental model whose value is in the elucidation of causal relationships related to human diabetes mellitus. Rats with blood glucose over 160 mgdl were considered as diabetic37. Amelioration of streptozotocin induced diabetes in rats by ecofriendly composite nanocinnamon extract. The introduction of chemical compounds in diabetes modeling cant adequately reflect the development of the disease. Jun 01, 2014 diabetes was induced in the 26 rats of dm groups by a single intraperitoneal injection of 60 mgkg body weight stz.
Glucoselowering and hypolipidemic activities of polysaccharides. Diabetes was induced by feeding high fat diet hfd for 2 weeks followed by intraperitoneal injection of streptozotocin 35 mgkg bwt. Effects of momordica charantia fruit juice on islet morphology in the pancreas of the streptozotocin diabetic rat. Group vinormal rats treated with poly herbal extra ct 200 mgkg, p. The mangiferin treated stz induced diabetic rats increased the level of gsh in liver and kidney tissues.
Diabetes was induced in seven equal groups of rats by a single intraperitoneal injection of streptozotocin 40 mgkg, in addition to the nondiabetic control group. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. Stz diabetic rats n96 showed stable signs of diabetes hyperglycaemia. Streptozotocininduced experimental diabetes in male. Effects of vascular injury on neointimal hyperplasia in rats with stz induced diabetes protocol i after stz injection, a 30% mortality was observed.
As alternative models of a type 2 diabetes in rodents also induce by streptozotocin injection and high fructose diet. This study was designed to investigate the preventive effects of metformin on stereological and ultrastructure characteristics of the ovary in the streptozotocin induced diabetes adult female rats. In the mid1960s, streptozotocin was found to be selectively toxic to the beta cells of the pancreatic islets, the cells that normally regulate blood glucose levels by producing the hormone insulin. Oxidative stress constitutes the key and common events in the pathogenesis of dn and antioxidants may play a beneficial role in its prevention. This unit describes protocols for the production of insulin deficiency and hyperglycemia in mice and rats, using stz. These animals were fed standard laboratory chow and a 5% glucose solution ad libitum for 72 h. Antidiabetic activity of crude polysaccharide and rhamnose. Antihyperglycemic effect of ginkgo biloba extract in. A reproducible neointimal formation was found 14 days after balloon injury in the control group con group, n12. Rings with and without endothelium were suspended in organ chambers for isometric tension recording. Pdf induction of diabetes by streptozotocin in rats researchgate.
The highest stz dose 70 mgkg was lethal to the animals, the doses of 50 and. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. Pdf the objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of. The mechanisms of alloxan and streptozotocininduced diabetes. Mice were treated with stz 40 mgkg or without stz vehicle control for 5 consecutive days. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Firstly, it closely resembles human type 1 diabetes with chronic pancreatic islet inflammation, insulitis, and insulin. Diabetes was induced by a single intravenous injection of streptozotocin stz in doses of 40, 50, and 60 mgkg in citrate buffer to 8weekold male rats, weighing 200230 g for the general procedure see the report by gajdosik et al 1999 published in this. Rats were made diabetic by injections of streptozotocin 75 mgikg, i. Typical changes in insulin secretion and blood glucose levels are illustrated. The animals were kept on a standard diet with free access to water for 4. Tigernut cyperus esculentus stzdiabetic rats lipid profiles. Treatment of streptozotocininduced diabetic rats with ave7688, a. Changes in oxidative stress and antioxidant enzyme activities in.
Again, this insulinopenia syndrome, called streptozotocin diabetes, is caused by the specific necrosis of the pancreatic beta cells and streptozotocin has been the agent of choice for the induction of diabetes mellitus in animals ever since 3, 16. Oxygenderived free radicals mediate endotheliumdependent contractions in femoral arteries of rats with streptozotocin induced diabetes. Both streptozotocin stz induced diabetic and type 2 diabetic t2d rats exhibited bone marrow neuropathy, which was concluded to contribute to diabetic retinopathy 14. Streptozotocin stzinduced diabetic model creative biolabs. Each tuber of yam was washed free of dirt, skinned and then cut to. Does honey have any salutary effect against streptozotocin. The aim of the present study was to investigate the beneficial effect of oral administration of mangiferin in streptozotocin stz induced diabetic rats by measuring the oxidative indicators in liver and kidney as well as the ameliorative properties. Feb 02, 2012 type i dm was induced in 20 rats by a double ip injection of stz sigma as 30 mg kg for two consecutive days with a total of 60 mg kg rat. Jan 01, 2019 this study investigated the effects of dapagliflozin, a sodium glucose cotransporter 2 inhibitor, on some early markers for dn in fructose streptozotocin fr stz induced diabetes in rats. Effects of streptozotocininduced diabetes on bladder and. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. Streptozotocininduced experimental diabetes in male wistar rats. The combined effect of low dose stz and high fructose diet allows in relatively short period induce the development of type 2 diabetes in rats. Hyperglycemia, hypoinsulinemia, polyphagia, polyuria and polydipsia accompanied by weight loss were seen in adult rats within three days of streptozotocin treatment and, within one week the amounts of the relevant factors were almost stable, which indicates irreversible destruction of.
In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to. Discussionthe results of this study have demonstrated that the diabetogenic agent stz can elevate blood glucose sugar within 24 h following injection and the blood glucose sugar remained elevated throughout the time course of the experiments compared to control rats. Mar 15, 2008 streptozotocin stz is an antibiotic that can cause pancreatic cell destruction, so it is widely used experimentally as an agent capable of inducing insulin. Research design and methods this study evaluated how stz induced diabetes affects adaptive immunity and the. Type i diabetes is also correlated with increased formation of free radicals and decreased levels of antioxidant potential. Body weights and blood glucose levels were monitored weekly. Mirza anwar baig and shital sharad panchal affiliation. In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to 70 mgkg rakieten et al. Streptozotocin induced diabetes mellitus in neonatal rats. Immunosuppressive effects of streptozotocininduced diabetes. Pdf alleviation of diabetic nephropathy by zinc oxide. Fr stz rats were treated with either dapagliflozin 1 mgkg p. Serum and liver lipids distributions in streptozotocin induced.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis. Effect of streptozotocin on glucose levels in albino. Cystometric studies were conducted on all rats, with cavernosometric studies conducted on a. Experimental diabetes was induced by a single intraperitoneal i. This study was conducted to investigate the effect of zinc chloride on streptozotocin induced diabetic nephropathy in rats compared to.
The increased consumption of high fatcontaining foods has been linked to the prevalence of obesity and abnormal metabolic syndromes. Induction of diabetes by streptozotocin in rats springerlink. After 10 days of biotelemetry data collection, diabetes was induced in five of the 10 rats by a single intraperitoneal injection of stz 60 mg kg body weight. Cerebellar synaptopathy in streptozotocininduced diabetic rats. Free radicals and antioxidants antioxidants are substances or nutrients in our foods, which can prevent or slow the. Stz is well known to cause pancreatic bcell damage, whereas na is administered to rats to partially protect insulinsecreting cells against stz. Dapagliflozin attenuates early markers of diabetic. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Jan 17, 2020 diabetes was induced in rats by a single intraperitoneal i.
Department of pharmcology, institute of pharmacy, nirma university, ahmedabad, gujarat, department of pharmcology, institute of pharmacy, nirma university, ahmedabad, gujarat. Immunosuppressive effects of streptozotocininduced. Diabetes was induced in male wistar rats by the administration of stz 60 mgkg b. Serum and liver lipids distributions in streptozotocin induced diabetic rat treated with diet. Black mulberry fruit extract alleviates streptozotocin. Streptozotocininduced diabetes mellitus in neonatal rats. In order to determine whether diabetic cardiomyopathy in rats is associated with altered contractile proteins, male and female rats were made diabetic with intravenous streptozotocin stz. The cytotoxic action of stz is mediated by free radicals and stz has toxic and carcinogenic effects on the pancreas, liver and kidneys okawa and doi, 1983. Rhizopus oligosporus, a fungus in the family mucoraceae, is widely used as a starter for homemade tempeh. The most widely used stz induced diabetic model is elicited in mice. Dec 20, 2012 the aim of this study was to evaluate the antihyperglycemic effects of gbe on streptozotocin stz induced diabetes in rats. Article in russian mazo vk, sidorova ys, zorin sn, kochetkova aa. Sd rats were used to induce diabetes using streptozotocin.
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